› 1j8h

X-ray diffraction
2.4Å resolution

Crystal Structure of a Complex of a Human alpha/beta-T cell Receptor, Influenza HA Antigen Peptide, and MHC Class II Molecule, HLA-DR4

Released:
DOI: 10.2210/pdb1j8h/pdb
Model geometry
Fit model/data
PDB entry 1j8h coloured by chain, front view.
PDB entry 1j8h coloured by chain, side view.
PDB entry 1j8h coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a complex of the human alpha/beta T cell receptor (TCR) HA1.7, influenza hemagglutinin peptide, and major histocompatibility complex class II molecule, HLA-DR4 (DRA*0101 and DRB1*0401): insight into TCR cross-restriction and alloreactivity.
Hennecke J, Wiley DC
OpenAccess logo J Exp Med 195 571-81 (2002)
PMID: 11877480

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134724 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01911 (Residues: 30-221; Coverage: 81%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Hemagglutinin HA1 chain Chain: C
T cell receptor alpha chain constant Chain: D
T cell receptor beta constant 1 Chain: E

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 143.753Å b: 73.317Å c: 123.033Å
α: 90° β: 108.52° γ: 90°
R-values:
R R work R free
0.211 0.211 0.246
Expression systems:
  • Drosophila melanogaster
  • Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
22 more

55 mentions without citation

The molecular basis of TCR germline bias for MHC is surprisingly simple.
Garcia et al. (2009)
54 more