› 1j8m

X-ray diffraction
2Å resolution

Signal Recognition Particle conserved GTPase domain from A. ambivalens

Released:
DOI: 10.2210/pdb1j8m/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1j8m coloured by chain, front view.
PDB entry 1j8m coloured by chain, side view.
PDB entry 1j8m coloured by chain, top view.
Source organism: Acidianus ambivalens
Primary publication:
The crystal structure of the conserved GTPase of SRP54 from the archaeon Acidianus ambivalens and its comparison with related structures suggests a model for the SRP-SRP receptor complex.
Montoya G, Kaat K, Moll R, Schäfer G, Sinning I
Structure 8 515-25 (2000)
PMID: 10801496

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle 54 kDa protein Chain: F
Molecule details ›
Chain: F
Length: 297 amino acids
Theoretical weight: 32.84 KDa
Source organism: Acidianus ambivalens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P70722 (Residues: 1-292; Coverage: 65%)
Gene names: ffh, srp54
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 64.866Å b: 128.02Å c: 72.004Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

The signal recognition particle.
Keenan et al. (2001)
11 more

7 mentions without citation

Archaea signal recognition particle shows the way.
Zwieb et al. (2010)
6 more