› 1j95

X-ray diffraction
2.8Å resolution

KCSA potassium channel with TBA (tetrabutylammonium) and potassium

Released:
DOI: 10.2210/pdb1j95/pdb
PDB entry 1j95 coloured by chain, front view.
PDB entry 1j95 coloured by chain, side view.
PDB entry 1j95 coloured by chain, top view.
Source organism: Streptomyces lividans
Primary publication:
Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.
Zhou M, Morais-Cabral JH, Mann S, MacKinnon R
Nature 411 657-61 (2001)
PMID: 11395760

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pH-gated potassium channel KcsA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.38 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-125; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand TBA 1 x TBA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2
Unit cell:
a: 128.9Å b: 67.4Å c: 112Å
α: 90° β: 125.3° γ: 90°
R-values:
R R work R free
0.32 0.298 0.322
Expression system: Escherichia coli

Quick links

Citations

66 review citations

The voltage-gated potassium channels and their relatives.
Yellen G. (2002)
65 more

20 mentions without citation

Orientation and dynamics of transmembrane peptides: the power of simple models.
Holt et al. (2010)
19 more