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X-ray diffraction
1.8Å resolution

Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)

Released:

Function and Biology Details

Reaction catalysed:
Reduced coenzyme F420 + NADP(+) = oxidized coenzyme F420 + NADPH
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-128119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F420-dependent NADP reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 22.89 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29370 (Residues: 1-212; Coverage: 100%)
Gene names: AF_0892, fno
Sequence domains: NADP oxidoreductase coenzyme F420-dependent
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 38.64Å b: 72.14Å c: 70.4Å
α: 90° β: 90.12° γ: 90°
R-values:
R R work R free
0.185 0.185 0.22
Expression system: Escherichia coli BL21(DE3)