› 1jbp

X-ray diffraction
2.2Å resolution

Crystal Structure of the Catalytic Subunit of cAMP-dependent Protein Kinase Complexed with a Substrate Peptide, ADP and Detergent

Released:
DOI: 10.2210/pdb1jbp/pdb
PDB entry 1jbp coloured by chain, front view.
PDB entry 1jbp coloured by chain, side view.
PDB entry 1jbp coloured by chain, top view.
Source organisms:
Primary publication:
cAMP-dependent protein kinase: crystallographic insights into substrate recognition and phosphotransfer.
Trafny EA, Xuong NH, Adams JA, Ten Eyck LF, Taylor SS, Sowadski JM
Protein Sci 3 176-87 (1994)
PMID: 8003955

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138545 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: S
Molecule details ›
Chain: S
Length: 20 amino acids
Theoretical weight: 2.2 KDa
Source organism: Oryctolagus cuniculus
Expression system: Not provided
UniProt:
  • Canonical: P61926 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand OCT 1 x OCT
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 73.96Å b: 76.11Å c: 81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

23 review citations

ERK1/2 MAP kinases: structure, function, and regulation.
Roskoski R. (2012)
22 more

19 mentions without citation

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
18 more