› 1jbw

X-ray diffraction
1.85Å resolution

FPGS-AMPPCP-folate complex

Released:
DOI: 10.2210/pdb1jbw/pdb
PDB entry 1jbw coloured by chain, front view.
PDB entry 1jbw coloured by chain, side view.
PDB entry 1jbw coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Folate-binding triggers the activation of folylpolyglutamate synthetase.
Sun X, Cross JA, Bognar AL, Baker EN, Smith CA
J Mol Biol 310 1067-78 (2001)
PMID: 11501996

Function and Biology Details

Reaction catalysed: 
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.7 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15925 (Residues: 1-428; Coverage: 100%)
Gene name: fpgS
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ACQ 1 x ACQ
Ligand TMF 1 x TMF
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 53.1Å b: 45.6Å c: 82.7Å
α: 90° β: 104.6° γ: 90°
R-values:
R R work R free
0.195 0.195 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
6 more

8 mentions without citation

FlexOracle: predicting flexible hinges by identification of stable domains.
Flores et al. (2007)
7 more