› 1jbz

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE ANALYSIS OF A DUAL-WAVELENGTH EMISSION GREEN FLUORESCENT PROTEIN VARIANT AT HIGH PH

Released:
DOI: 10.2210/pdb1jbz/pdb
PDB entry 1jbz coloured by chain, front view.
PDB entry 1jbz coloured by chain, side view.
PDB entry 1jbz coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application.
Hanson GT, McAnaney TB, Park ES, Rendell ME, Yarbrough DK, Chu S, Xi L, Boxer SG, Montrose MH, Remington SJ
Biochemistry 41 15477-88 (2002)
PMID: 12501176

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.87 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand EDO 3 x EDO
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.2Å b: 62.374Å c: 68.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 not available
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Nonlinear magic: multiphoton microscopy in the biosciences.
Zipfel et al. (2003)
43 more

3 mentions without citation

Specific GFP-binding artificial proteins (αRep): a new tool for in vitro to live cell applications.
Chevrel et al. (2015)
2 more