› 1jfz

X-ray diffraction
2.1Å resolution

Crystal Structure of MN(II)-Complex of RNAse III Endonuclease Domain from Aquifex Aeolicus at 2.10 Angstrom Resolution

Released:
DOI: 10.2210/pdb1jfz/pdb
PDB entry 1jfz coloured by chain, front view.
PDB entry 1jfz coloured by chain, side view.
PDB entry 1jfz coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.
Blaszczyk J, Tropea JE, Bubunenko M, Routzahn KM, Waugh DS, Court DL, Ji X
Structure 9 1225-36 (2001)
PMID: 11738048

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 18.16 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67082 (Residues: 1-147; Coverage: 67%)
Gene names: aq_946, rnc
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease III domain

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 49.741Å b: 140.549Å c: 49.759Å
α: 90° β: 117.42° γ: 90°
R-values:
R R work R free
0.208 0.195 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

45 review citations

MicroRNAs: small RNAs with a big role in gene regulation.
He et al. (2004)
44 more

5 mentions without citation

Comparative analysis of editosome proteins in trypanosomatids.
Worthey et al. (2003)
4 more