› 1jh7

X-ray diffraction
2.4Å resolution

Semi-reduced Inhibitor-bound Cyclic Nucleotide Phosphodiesterase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb1jh7/pdb
PDB entry 1jh7 coloured by chain, front view.
PDB entry 1jh7 coloured by chain, side view.
PDB entry 1jh7 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of the semireduced and inhibitor-bound forms of cyclic nucleotide phosphodiesterase from Arabidopsis thaliana.
Hofmann A, Grella M, Botos I, Filipowicz W, Wlodawer A
J Biol Chem 277 1419-25 (2002)
PMID: 11694509

Function and Biology Details

Reaction catalysed: 
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 2'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.51 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O04147 (Residues: 1-181; Coverage: 100%)
Gene names: At4g18930, F13C5.100, F13C5_100
Sequence domains: Cyclic phosphodiesterase-like protein
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand UVC 2 x UVC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: R32
Unit cell:
a: 101.14Å b: 101.14Å c: 90Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.399
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes.
Davies et al. (2004)
1 more

4 mentions without citation

A robust bulk-solvent correction and anisotropic scaling procedure.
Afonine et al. (2005)
3 more