› 1ji3

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE FIRST THERMOSTABLE BACTERIAL LIPASE FROM BACILLUS STEAROTHERMOPHILUS

Released:
DOI: 10.2210/pdb1ji3/pdb
PDB entry 1ji3 coloured by chain, front view.
PDB entry 1ji3 coloured by chain, side view.
PDB entry 1ji3 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of a thermostable lipase from Bacillus stearothermophilus P1.
Tyndall JD, Sinchaikul S, Fothergill-Gilmore LA, Taylor P, Walkinshaw MD
J Mol Biol 323 859-69 (2002)
PMID: 12417199

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.31 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L6D3 (Residues: 30-417; Coverage: 93%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2
Unit cell:
a: 118.5Å b: 81.237Å c: 99.782Å
α: 90° β: 96.33° γ: 90°
R-values:
R R work R free
0.169 0.166 0.21
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
4 more

9 mentions without citation

The multiple roles of histidine in protein interactions.
Liao et al. (2013)
8 more