› 1jic

Solution NMR

SOLUTION NMR STRUCTURE OF RECOMBINANT SSO7D WITH RNASE ACTIVITY, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1jic/pdb
PDB entry 1jic coloured by chain, front view.
PDB entry 1jic coloured by chain, side view.
PDB entry 1jic coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core.
Consonni R, Santomo L, Fusi P, Tortora P, Zetta L
Biochemistry 38 12709-17 (1999)
PMID: 10504241

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein 7a Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.03 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61991 (Residues: 2-63; Coverage: 97%)
Gene names: SSO9180, SSO9535, sso7a1, sso7a2, sso7d-2, sso7d-3
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein adaptations in archaeal extremophiles.
Reed et al. (2013)
1 more

4 mentions without citation

Advances in Human Immune System Mouse Models for Studying Human Hematopoiesis and Cancer Immunotherapy.
Mian et al. (2020)
3 more