› 1jj4

X-ray diffraction
2.4Å resolution

Human papillomavirus type 18 E2 DNA-binding domain bound to its DNA target

Released:
DOI: 10.2210/pdb1jj4/pdb
PDB entry 1jj4 coloured by chain, front view.
PDB entry 1jj4 coloured by chain, side view.
PDB entry 1jj4 coloured by chain, top view.
Source organism: human papillomavirus 18
Primary publication:
The structural basis of DNA target discrimination by papillomavirus E2 proteins.
Kim SS, Tam JK, Wang AF, Hegde RS
J Biol Chem 275 31245-54 (2000)
PMID: 10906136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114008 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein E2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 83 amino acids
Theoretical weight: 9.59 KDa
Source organism: Human papillomavirus type 18
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06790 (Residues: 286-365; Coverage: 22%)
Gene name: E2
Sequence domains: E2 (early) protein, C terminal
Structure domains: Alpha-Beta Plaits
E2 binding site DNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: human papillomavirus 18
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 69.88Å b: 82.19Å c: 96.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Human papillomaviruses: basic mechanisms of pathogenesis and oncogenicity.
Hebner et al. (2006)
5 more

25 mentions without citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
24 more