› 1jji

X-ray diffraction
2.2Å resolution

The Crystal Structure of a Hyper-thermophilic Carboxylesterase from the Archaeon Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb1jji/pdb
PDB entry 1jji coloured by chain, front view.
PDB entry 1jji coloured by chain, side view.
PDB entry 1jji coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
The crystal structure of a hyper-thermophilic carboxylesterase from the archaeon Archaeoglobus fulgidus.
De Simone G, Menchise V, Manco G, Mandrich L, Sorrentino N, Lang D, Rossi M, Pedone C
J Mol Biol 314 507-18 (2001)
PMID: 11846563

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-128038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 35.53 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28558 (Residues: 1-311; Coverage: 100%)
Gene name: AF_1716
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand EPE 4 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P62
Unit cell:
a: 169.05Å b: 169.05Å c: 104.544Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Stabilizing biocatalysts.
Bommarius et al. (2013)
4 more

27 mentions without citation

Carboxylic ester hydrolases from hyperthermophiles.
Levisson et al. (2009)
26 more