› 1jjw

X-ray diffraction
1.9Å resolution

Structure of Haemophilus influenzae HslV Protein at 1.9 A Resolution

Released:
DOI: 10.2210/pdb1jjw/pdb
PDB entry 1jjw coloured by chain, front view.
PDB entry 1jjw coloured by chain, side view.
PDB entry 1jjw coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structure of Haemophilus influenzae HslV protein at 1.9 A resolution, revealing a cation-binding site near the catalytic site.
Sousa MC, McKay DB
Acta Crystallogr D Biol Crystallogr 57 1950-4 (2001)
PMID: 11717526

Function and Biology Details

Reaction catalysed: 
ATP-dependent cleavage of peptide bonds with broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155127 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease subunit HslV Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 18.9 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P43772 (Residues: 2-175; Coverage: 99%)
Gene names: HI_0496, hslV
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 78.893Å b: 122.331Å c: 126.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.249
Expression system: Escherichia coli BL21

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Citations

3 review citations

Molecular machines for protein degradation.
Groll et al. (2005)
2 more