› 1jkx

X-ray diffraction
1.6Å resolution

Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase

Released:
DOI: 10.2210/pdb1jkx/pdb
PDB entry 1jkx coloured by chain, front view.
PDB entry 1jkx coloured by chain, side view.
PDB entry 1jkx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase.
Greasley SE, Marsilje TH, Cai H, Baker S, Benkovic SJ, Boger DL, Wilson IA
Biochemistry 40 13538-47 (2001)
PMID: 11695901

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08179 (Residues: 1-212; Coverage: 100%)
Gene names: JW2485, b2500, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 138 4 x 138
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P1
Unit cell:
a: 55.14Å b: 56.01Å c: 76.13Å
α: 80.83° β: 71.71° γ: 83.69°
R-values:
R R work R free
0.221 0.221 0.243
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
4 more

3 mentions without citation

Biological and structural evaluation of 10R- and 10S-methylthio-DDACTHF reveals a new role for sulfur in inhibition of glycinamide ribonucleotide transformylase.
Connelly et al. (2013)
2 more