› 1jml

X-ray diffraction
1.9Å resolution

Conversion of Monomeric Protein L to an Obligate Dimer by Computational Protein Design

Released:
DOI: 10.2210/pdb1jml/pdb
PDB entry 1jml coloured by chain, front view.
PDB entry 1jml coloured by chain, side view.
PDB entry 1jml coloured by chain, top view.
Source organism: Finegoldia magna ATCC 29328
Primary publication:
Conversion of monomeric protein L to an obligate dimer by computational protein design.
Kuhlman B, O'Neill JW, Kim DE, Zhang KY, Baker D
Proc Natl Acad Sci U S A 98 10687-91 (2001)
PMID: 11526208

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-175983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 8.06 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51912 (Residues: 111-173; Coverage: 9%)
Sequence domains: Protein L b1 domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 46.762Å b: 76.744Å c: 59.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
13 more

2 mentions without citation

Conversion of monomeric protein L to an obligate dimer by computational protein design.
Kuhlman et al. (2001)
1 more