› 1jmu

X-ray diffraction
2.8Å resolution

Crystal Structure of the Reovirus mu1/sigma3 Complex

Released:
DOI: 10.2210/pdb1jmu/pdb
PDB entry 1jmu coloured by chain, front view.
PDB entry 1jmu coloured by chain, side view.
PDB entry 1jmu coloured by chain, top view.
Source organism: Reovirus sp.
Primary publication:
Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3.
Liemann S, Chandran K, Baker TS, Nibert ML, Harrison SC
Cell 108 283-95 (2002)
PMID: 11832217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139843 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Outer capsid protein mu-1N Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 41 amino acids
Theoretical weight: 4.05 KDa
Source organism: Reovirus sp.
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11077 (Residues: 2-42; Coverage: 6%)
Gene name: M2
Outer capsid protein mu-1 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 666 amino acids
Theoretical weight: 72.18 KDa
Source organism: Reovirus sp.
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11077 (Residues: 43-708; Coverage: 94%)
Gene name: M2
Sequence domains: Reovirus major virion structural protein Mu-1/Mu-1C (M2)
Structure domains:
Outer capsid protein sigma-3 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 366 amino acids
Theoretical weight: 41.26 KDa
Source organism: Reovirus sp.
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07939 (Residues: 1-365; Coverage: 100%)
Gene name: S4
Sequence domains: Reovirus outer capsid protein, Sigma 3
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand BOG 3 x BOG
Ligand SO4 9 x SO4
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I222
Unit cell:
a: 180.294Å b: 184.942Å c: 284.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.241
Expression system: Spodoptera frugiperda

Quick links

Citations

31 review citations

Viral and cellular determinants involved in hepadnaviral entry.
Glebe et al. (2007)
30 more

22 mentions without citation

Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3.
Liemann et al. (2002)
21 more