› 1jn4

X-ray diffraction
1.8Å resolution

The Crystal Structure of Ribonuclease A in complex with 2'-deoxyuridine 3'-pyrophosphate (P'-5') adenosine

Released:
DOI: 10.2210/pdb1jn4/pdb
PDB entry 1jn4 coloured by chain, front view.
PDB entry 1jn4 coloured by chain, side view.
PDB entry 1jn4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Cleavage of 3',5'-pyrophosphate-linked dinucleotides by ribonuclease A and angiogenin.
Jardine AM, Leonidas DD, Jenkins JL, Park C, Raines RT, Acharya KR, Shapiro R
Biochemistry 40 10262-72 (2001)
PMID: 11513604

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand 139 1 x 139
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: C2
Unit cell:
a: 99.822Å b: 32.291Å c: 72.061Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.225 0.225 0.295

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Citations

14 citation in other articles

A small-molecule inhibitor of the ribonucleolytic activity of human angiogenin that possesses antitumor activity.
Kao et al. (2002)
13 more

9 mentions without citation

Hypervalent nonbonded interactions of a divalent sulfur atom. Implications in protein architecture and the functions.
Iwaoka et al. (2012)
8 more