› 1joe

X-ray diffraction
2.4Å resolution

Crystal Structure of Autoinducer-2 Production Protein (LuxS) from Heamophilus influenzae

Released:
DOI: 10.2210/pdb1joe/pdb
PDB entry 1joe coloured by chain, front view.
PDB entry 1joe coloured by chain, side view.
PDB entry 1joe coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Entry authors: Chen CCH, Parsons JF, Lim K, Lehmann C, Tempczyk A, Eisenstein E, Herzberg O, Structure 2 Function Project (S2F)

Function and Biology Details

Reaction catalysed: 
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-ribosylhomocysteine lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 167 amino acids
Theoretical weight: 18.55 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P44007 (Residues: 1-167; Coverage: 100%)
Gene names: HI_0491, luxS
Sequence domains: S-Ribosylhomocysteinase (LuxS)
Structure domains: S-ribosylhomocysteinase (LuxS)

Ligands and Environments

2 bound ligands:
Ligand HG 4 x HG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P42
Unit cell:
a: 115.53Å b: 115.53Å c: 51.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.205 0.299
Expression system: Escherichia coli

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