› 1jpu

X-ray diffraction
1.8Å resolution

Crystal Structure of Bacillus Stearothermophilus Glycerol Dehydrogenase

Released:
DOI: 10.2210/pdb1jpu/pdb
PDB entry 1jpu coloured by chain, front view.
PDB entry 1jpu coloured by chain, side view.
PDB entry 1jpu coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Glycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.
Ruzheinikov SN, Burke J, Sedelnikova S, Baker PJ, Taylor R, Bullough PA, Muir NM, Gore MG, Rice DW
Structure 9 789-802 (2001)
PMID: 11566129

Function and Biology Details

Reaction catalysed: 
Glycerol + NAD(+) = glycerone + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 39.56 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32816 (Residues: 1-370; Coverage: 100%)
Gene names: gld, gldA
Sequence domains: Iron-containing alcohol dehydrogenase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I422
Unit cell:
a: 105.03Å b: 105.03Å c: 148.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 0.208
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Key enzymes catalyzing glycerol to 1,3-propanediol.
Jiang et al. (2016)
2 more

4 mentions without citation

1,3-Propanediol dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity.
Marçal et al. (2009)
3 more