› 1jq6

X-ray diffraction
2.3Å resolution

HUMAN CYTOMEGALOVIRUS PROTEASE DIMER-INTERFACE MUTANT, S225Y

Released:
DOI: 10.2210/pdb1jq6/pdb
PDB entry 1jq6 coloured by chain, front view.
PDB entry 1jq6 coloured by chain, side view.
PDB entry 1jq6 coloured by chain, top view.
Source organism: Human betaherpesvirus 5
Primary publication:
Molecular mechanism for dimerization to regulate the catalytic activity of human cytomegalovirus protease.
Batra R, Khayat R, Tong L
Nat Struct Biol 8 810-7 (2001)
PMID: 11524687

Function and Biology Details

Reaction catalysed: 
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Assemblin Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.52 KDa
Source organism: Human betaherpesvirus 5
Expression system: Escherichia coli
UniProt:
  • Canonical: P16753 (Residues: 1-256; Coverage: 36%)
Gene names: APNG, UL80
Sequence domains: Assemblin (Peptidase family S21)
Structure domains: Herpesvirus/Caudovirus protease domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CAS 3 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 42.27Å b: 108.3Å c: 108.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.229 0.286
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Non-nucleoside inhibitors of herpesviruses.
Wathen MW. (2002)
5 more

1 mention without citation

Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.
Zühlsdorf et al. (2015)