› 1jqb

X-ray diffraction
1.97Å resolution

Alcohol Dehydrogenase from Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability

Released:
DOI: 10.2210/pdb1jqb/pdb
PDB entry 1jqb coloured by chain, front view.
PDB entry 1jqb coloured by chain, side view.
PDB entry 1jqb coloured by chain, top view.
Source organism: Clostridium beijerinckii
Primary publication:
Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging.
Bogin O, Levin I, Hacham Y, Tel-Or S, Peretz M, Frolow F, Burstein Y
Protein Sci 11 2561-74 (2002)
PMID: 12381840

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 37.86 KDa
Source organism: Clostridium beijerinckii
Expression system: Escherichia coli
UniProt:
  • Canonical: P25984 (Residues: 1-351; Coverage: 100%)
Gene name: adh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 86.7Å b: 147.8Å c: 125.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.247
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New understandings of thermostable and peizostable enzymes.
Yano et al. (2003)
1 more

2 mentions without citation

Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein.
Park et al. (2006)
1 more