› 1jqc

X-ray diffraction
1.61Å resolution

Mn substituted Ribonucleotide reductase R2 from E. Coli oxidized by hydrogen peroxide and hydroxylamine

Released:
DOI: 10.2210/pdb1jqc/pdb
PDB entry 1jqc coloured by chain, front view.
PDB entry 1jqc coloured by chain, side view.
PDB entry 1jqc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of oxidized dinuclear manganese centres in Mn-substituted class I ribonucleotide reductase from Escherichia coli: carboxylate shifts with implications for O2 activation and radical generation.
Högbom M, Andersson ME, Nordlund P
J Biol Inorg Chem 6 315-23 (2001)
PMID: 11315567

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase 1 subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 43.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69924 (Residues: 2-376; Coverage: 100%)
Gene names: JW2229, b2235, ftsB, nrdB
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand HG 13 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P212121
Unit cell:
a: 73.848Å b: 84.554Å c: 114.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of the radical enzyme ribonucleotide reductase.
Eklund et al. (2001)
1 more

2 mentions without citation

Equilibration of tyrosyl radicals (Y356•, Y731•, Y730•) in the radical propagation pathway of the Escherichia coli class Ia ribonucleotide reductase.
Yokoyama et al. (2011)
1 more