› 1jr7

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF GAB REVEALS OXIDOREDUCTASE FOLD

Released:
DOI: 10.2210/pdb1jr7/pdb
PDB entry 1jr7 coloured by chain, front view.
PDB entry 1jr7 coloured by chain, side view.
PDB entry 1jr7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural genomics: a pipeline for providing structures for the biologist.
Chance MR, Bresnick AR, Burley SK, Jiang JS, Lima CD, Sali A, Almo SC, Bonanno JB, Buglino JA, Boulton S, Chen H, Eswar N, He G, Huang R, Ilyin V, McMahan L, Pieper U, Ray S, Vidal M, Wang LK
Protein Sci 11 723-38 (2002)
PMID: 11910018

Function and Biology Details

Reaction catalysed: 
Glutarate + 2-oxoglutarate + O(2) = (S)-2-hydroxyglutarate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutarate 2-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 35.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76621 (Residues: 15-325; Coverage: 96%)
Gene names: JW5427, b2659, csiD, gab, glaH, ygaT
Sequence domains: CsiD
Structure domains: Clavaminate synthase-like

Ligands and Environments

1 bound ligand:
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I422
Unit cell:
a: 120.479Å b: 120.479Å c: 136.604Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Alkylation damage in DNA and RNA--repair mechanisms and medical significance.
Drabløs et al. (2004)
19 more

6 mentions without citation

Structural genomics: a pipeline for providing structures for the biologist.
Chance et al. (2002)
5 more