› 1jri

X-ray diffraction
2.8Å resolution

The Crystal Structure of an Sm-like Archaeal Protein with Two Heptamers in the Asymmetric Unit.

Released:
DOI: 10.2210/pdb1jri/pdb
PDB entry 1jri coloured by chain, front view.
PDB entry 1jri coloured by chain, side view.
PDB entry 1jri coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs).
Mura C, Kozhukhovsky A, Gingery M, Phillips M, Eisenberg D
Protein Sci 12 832-47 (2003)
PMID: 12649441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative snRNP Sm-like protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 85 amino acids
Theoretical weight: 9.5 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O26745 (Residues: 1-81; Coverage: 100%)
Gene name: MTH_649
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand EDO 13 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 40.371Å b: 114.698Å c: 238.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Hfq structure, function and ligand binding.
Brennan et al. (2007)
6 more

2 mentions without citation

The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs).
Mura et al. (2003)
1 more