› 1jrk

X-ray diffraction
2.4Å resolution

Crystal Structure of a Nudix Protein from Pyrobaculum aerophilum Reveals a Dimer with Intertwined Beta Sheets

Released:
DOI: 10.2210/pdb1jrk/pdb
PDB entry 1jrk coloured by chain, front view.
PDB entry 1jrk coloured by chain, side view.
PDB entry 1jrk coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
Structure of a Nudix protein from Pyrobaculum aerophilum reveals a dimer with two intersubunit beta-sheets.
Wang S, Mura C, Sawaya MR, Cascio D, Eisenberg D
Acta Crystallogr D Biol Crystallogr 58 571-8 (2002)
PMID: 11914479

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-187402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nudix hydrolase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 156 amino acids
Theoretical weight: 17.81 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZTD8 (Residues: 6-146; Coverage: 97%)
Gene name: PAE3301
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand MPD 6 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P21
Unit cell:
a: 52.469Å b: 81.464Å c: 73.888Å
α: 90° β: 99.96° γ: 90°
R-values:
R R work R free
0.19 0.19 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structures and mechanisms of Nudix hydrolases.
Mildvan et al. (2005)
1 more

2 mentions without citation

De-orphaning the structural proteome through reciprocal comparison of evolutionarily important structural features.
Ward et al. (2008)
1 more