› 1jst

X-ray diffraction
2.6Å resolution

PHOSPHORYLATED CYCLIN-DEPENDENT KINASE-2 BOUND TO CYCLIN A

Released:
DOI: 10.2210/pdb1jst/pdb
PDB entry 1jst coloured by chain, front view.
PDB entry 1jst coloured by chain, side view.
PDB entry 1jst coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of cyclin-dependent kinase activation by phosphorylation.
Russo AA, Jeffrey PD, Pavletich NP
Nat Struct Biol 3 696-700 (1996)
PMID: 8756328

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148886 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 298 amino acids
Theoretical weight: 34.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 258 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 175-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand ATP 2 x ATP
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 139.6Å b: 149.1Å c: 74.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 not available
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

83 review citations

Cyclin-dependent kinases: engines, clocks, and microprocessors.
Morgan DO. (1997)
82 more

33 mentions without citation

Cyclin-dependent kinases.
Malumbres M. (2014)
32 more