› 1juv

X-ray diffraction
1.7Å resolution

Crystal structure analysis of Dihydrofolate reductase from Bacteriophage T4

Released:
DOI: 10.2210/pdb1juv/pdb
PDB entry 1juv coloured by chain, front view.
PDB entry 1juv coloured by chain, side view.
PDB entry 1juv coloured by chain, top view.
Source organism: Escherichia virus T4
Entry authors: Moon JH, Oh YK, Suh SW

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.74 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04382 (Residues: 1-193; Coverage: 100%)
Gene name: frd
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P4322
Unit cell:
a: 61.16Å b: 61.16Å c: 124.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.227 0.269
Expression system: Escherichia coli

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