› 1jv6

X-ray diffraction
2Å resolution

BACTERIORHODOPSIN D85S/F219L DOUBLE MUTANT AT 2.00 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1jv6/pdb
PDB entry 1jv6 coloured by chain, front view.
PDB entry 1jv6 coloured by chain, side view.
PDB entry 1jv6 coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate.
Rouhani S, Cartailler JP, Facciotti MT, Walian P, Needleman R, Lanyi JK, Glaeser RM, Luecke H
J Mol Biol 313 615-28 (2001)
PMID: 11676543

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.87 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand LI1 8 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 51.8Å b: 121.3Å c: 85.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.222 0.24
Expression system: Halobacterium salinarum

Quick links

Citations

9 review citations

Bacteriorhodopsin.
Lanyi JK. (2004)
8 more

9 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
8 more