› 1jvl

X-ray diffraction
2Å resolution

Azurin dimer, covalently crosslinked through bis-maleimidomethylether

Released:
DOI: 10.2210/pdb1jvl/pdb
PDB entry 1jvl coloured by chain, front view.
PDB entry 1jvl coloured by chain, side view.
PDB entry 1jvl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Dramatic modulation of electron transfer in protein complexes by crosslinking.
van Amsterdam IM, Ubbink M, Einsle O, Messerschmidt A, Merli A, Cavazzini D, Rossi GL, Canters GW
Nat Struct Biol 9 48-52 (2002)
PMID: 11740504

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 13.95 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NI 2 x NI
Ligand 144 2 x 144
Ligand OPP 1 x OPP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P61
Unit cell:
a: 48.605Å b: 48.605Å c: 284.809Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.227
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Paramagnetic resonance of biological metal centers.
Ubbink et al. (2002)
3 more

2 mentions without citation

Nanoscale dewetting transition in protein complex folding.
Hua et al. (2007)
1 more