› 1jvy

X-ray diffraction
1.9Å resolution

Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides

Released:
DOI: 10.2210/pdb1jvy/pdb
PDB entry 1jvy coloured by chain, front view.
PDB entry 1jvy coloured by chain, side view.
PDB entry 1jvy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystine: fibrous biomolecular material from protein crystals cross-linked in a specific geometry.
Srinivasan U, Iyer GH, Przybycien TA, Samsonoff WA, Bell JA
Protein Eng 15 895-902 (2002)
PMID: 12538909

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 41.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-326, 327-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 103.2Å b: 68.1Å c: 56.34Å
α: 90° β: 112.46° γ: 90°
R-values:
R R work R free
0.25 0.207 0.273
Expression system: Escherichia coli

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