› 1jx6

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2

Released:
DOI: 10.2210/pdb1jx6/pdb
PDB entry 1jx6 coloured by chain, front view.
PDB entry 1jx6 coloured by chain, side view.
PDB entry 1jx6 coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Structural identification of a bacterial quorum-sensing signal containing boron.
Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, Bassler BL, Hughson FM
Nature 415 545-9 (2002)
PMID: 11823863

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer 2-binding periplasmic protein LuxP Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 39.07 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54300 (Residues: 24-365; Coverage: 100%)
Gene name: luxP
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand AI2 1 x AI2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 44.268Å b: 77.467Å c: 52.008Å
α: 90° β: 96.37° γ: 90°
R-values:
R R work R free
0.216 0.214 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

230 review citations

Quorum sensing: cell-to-cell communication in bacteria.
Waters et al. (2005)
229 more

16 mentions without citation

Quorum Sensing Inhibitors: Curbing Pathogenic Infections through Inhibition of Bacterial Communication.
Singh et al. (2021)
15 more