› 1jxe

X-ray diffraction
2.85Å resolution

STOFFEL FRAGMENT OF TAQ DNA POLYMERASE I

Released:
DOI: 10.2210/pdb1jxe/pdb
PDB entry 1jxe coloured by chain, front view.
PDB entry 1jxe coloured by chain, side view.
PDB entry 1jxe coloured by chain, top view.
Source organism: Thermus aquaticus
Entry authors: Oefner C, Gelfand DH, Stoffel S, Winkler FK

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 60.94 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 293-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 110.1Å b: 137.8Å c: 45.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 not available
Expression system: Escherichia coli

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