› 1jyh

X-ray diffraction
1.8Å resolution

Crystal Structure of the Escherichia coli SbmC protein (AKA Gyrase Inhibitory Protein GyrI, AKA YeeB)

Released:
DOI: 10.2210/pdb1jyh/pdb
PDB entry 1jyh coloured by chain, front view.
PDB entry 1jyh coloured by chain, side view.
PDB entry 1jyh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the Escherichia coli SbmC protein that protects cells from the DNA replication inhibitor microcin B17.
Romanowski MJ, Gibney SA, Burley SK
Proteins 47 403-7 (2002)
PMID: 11948793

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase inhibitor Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P33012 (Residues: 1-157; Coverage: 100%)
Gene names: JW1991, b2009, gyrI, sbmC, yeeB
Sequence domains: Integron-associated effector binding protein
Structure domains: Regulatory factor, effector binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P43212
Unit cell:
a: 72.44Å b: 72.44Å c: 80.891Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.222
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.
Collin et al. (2011)
4 more

7 mentions without citation

Bacterial MerR family transcription regulators: activationby distortion.
Fang et al. (2022)
6 more