› 1jyq

X-ray diffraction
2Å resolution

Xray Structure of Grb2 SH2 Domain Complexed with a Highly Affine Phospho Peptide

Released:
DOI: 10.2210/pdb1jyq/pdb
PDB entry 1jyq coloured by chain, front view.
PDB entry 1jyq coloured by chain, side view.
PDB entry 1jyq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the SH2 domain of Grb2: highlight on the binding of a new high-affinity inhibitor.
Nioche P, Liu WQ, Broutin I, Charbonnier F, Latreille MT, Vidal M, Roques B, Garbay C, Ducruix A
J Mol Biol 315 1167-77 (2002)
PMID: 11827484

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-158875 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62993 (Residues: 60-151; Coverage: 42%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SH2 domain
mAZ-pY-(alpha Me)pY-N-NH2 peptide inhibitor Chains: H, L
Molecule details ›
Chains: H, L
Length: 4 amino acids
Theoretical weight: 766 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand PTR 2 x PTR
Ligand PTM 2 x PTM

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P6122
Unit cell:
a: 60.999Å b: 60.999Å c: 177.046Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Structural systems biology: modelling protein interactions.
Aloy et al. (2006)
3 more

4 mentions without citation

EGF-receptor specificity for phosphotyrosine-primed substrates provides signal integration with Src.
Begley et al. (2015)
3 more