› 1jzj

X-ray diffraction
1.8Å resolution

Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83)

Released:
DOI: 10.2210/pdb1jzj/pdb
PDB entry 1jzj coloured by chain, front view.
PDB entry 1jzj coloured by chain, side view.
PDB entry 1jzj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Electron tunneling in single crystals of Pseudomonas aeruginosa azurins.
Crane BR, Di Bilio AJ, Winkler JR, Gray HB
J Am Chem Soc 123 11623-31 (2001)
PMID: 11716717

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand CU 3 x CU
Ligand DOS 2 x DOS
Ligand LOS 2 x LOS
Ligand IME 1 x IME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 54.873Å b: 54.873Å c: 133.813Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Copper in diseases and treatments, and copper-based anticancer strategies.
Tisato et al. (2010)
11 more