› 1k0c

X-ray diffraction
2.5Å resolution

Ure2p in complex with S-p-nitrobenzylglutathione

Released:
DOI: 10.2210/pdb1k0c/pdb
PDB entry 1k0c coloured by chain, front view.
PDB entry 1k0c coloured by chain, side view.
PDB entry 1k0c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of the yeast prion Ure2p functional region in complex with glutathione and related compounds.
Bousset L, Belrhali H, Melki R, Morera S
Biochemistry 40 13564-73 (2001)
PMID: 11695904

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator URE2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 260 amino acids
Theoretical weight: 29.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P23202 (Residues: 95-354; Coverage: 73%)
Gene names: N1165, URE2, YNL229C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTB 3 x GTB

Cofactor: Ligand GSH 1 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 54.736Å b: 123.214Å c: 168.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.267
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Prions in yeast.
Liebman et al. (2012)
9 more