› 1k0j

X-ray diffraction
2.2Å resolution

Pseudomonas aeruginosa phbh R220Q in complex with NADPH and free of p-OHB

Released:
DOI: 10.2210/pdb1k0j/pdb
PDB entry 1k0j coloured by chain, front view.
PDB entry 1k0j coloured by chain, side view.
PDB entry 1k0j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase.
Wang J, Ortiz-Maldonado M, Entsch B, Massey V, Ballou D, Gatti DL
Proc Natl Acad Sci U S A 99 608-13 (2002)
PMID: 11805318

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.35 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P20586 (Residues: 1-394; Coverage: 100%)
Gene names: PA0247, pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP

Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 71.845Å b: 139.146Å c: 91.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.275
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
10 more

7 mentions without citation

MICAL, the flavoenzyme participating in cytoskeleton dynamics.
Vanoni et al. (2013)
6 more