› 1k0r

X-ray diffraction
1.7Å resolution

Crystal Structure of Mycobacterium tuberculosis NusA

Released:
DOI: 10.2210/pdb1k0r/pdb
PDB entry 1k0r coloured by chain, front view.
PDB entry 1k0r coloured by chain, side view.
PDB entry 1k0r coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of the transcription elongation/anti-termination factor NusA from Mycobacterium tuberculosis at 1.7 A resolution.
Gopal B, Haire LF, Gamblin SJ, Dodson EJ, Lane AN, Papavinasasundaram KG, Colston MJ, Dodson G
J Mol Biol 314 1087-95 (2001)
PMID: 11743725

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusA Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 39.87 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIV3 (Residues: 1-347; Coverage: 100%)
Gene names: MTCY16B7.01, MTCY24A1.16, Rv2841c, nusA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 88.987Å b: 88.987Å c: 180.208Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.248 0.271
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Structure and function of KH domains.
Valverde et al. (2008)
9 more

6 mentions without citation

Mycobacterial RNA polymerase requires a U-tract at intrinsic terminators and is aided by NusG at suboptimal terminators.
Czyz et al. (2014)
5 more