› 1k0z

X-ray diffraction
2.05Å resolution

Crystal Structure of the PvuII endonuclease with Pr3+ and SO4 ions bound in the active site at 2.05A.

Released:
DOI: 10.2210/pdb1k0z/pdb
PDB entry 1k0z coloured by chain, front view.
PDB entry 1k0z coloured by chain, side view.
PDB entry 1k0z coloured by chain, top view.
Source organism: Proteus vulgaris
Primary publication:
Crystal Structure of PvuII endonuclease reveals extensive structural homologies to EcoRV
Athanasiadis A, Vlassi M, Kotsifaki D, Tucker PA, Wilson KS, Kokkinidis M
Nat. Struct. Biol. 1 469-475 (1994)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme PvuII Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 18.24 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P23657 (Residues: 2-157; Coverage: 99%)
Gene name: pvuIIR
Sequence domains: Restriction endonuclease PvuII
Structure domains: PVUII Endonuclease, subunit A

Ligands and Environments

2 bound ligands:
Ligand PR 2 x PR
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 84.74Å b: 105.63Å c: 46.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.236
Expression system: Escherichia coli

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