1k1g

Solution NMR

STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172355 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor 1 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15637 (Residues: 133-260; Coverage: 20%)
Gene names: SF1, ZFM1, ZNF162
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
5'-R(*UP*AP*UP*AP*CP*UP*AP*AP*CP*AP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: restrained molecular dynamics, simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided