› 1k1q

X-ray diffraction
2.8Å resolution

Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb1k1q/pdb
PDB entry 1k1q coloured by chain, front view.
PDB entry 1k1q coloured by chain, side view.
PDB entry 1k1q coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus.
Silvian LF, Toth EA, Pham P, Goodman MF, Ellenberger T
Nat Struct Biol 8 984-9 (2001)
PMID: 11685247

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 40.03 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P96022 (Residues: 1-354; Coverage: 100%)
Gene name: dbh
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P61
Unit cell:
a: 111.715Å b: 111.715Å c: 132.175Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.263 0.306
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
24 more