› 1k26

X-ray diffraction
1.85Å resolution

Structure of a Nudix Protein from Pyrobaculum aerophilum Solved by the Single Wavelength Anomolous Scattering Method

Released:
DOI: 10.2210/pdb1k26/pdb
PDB entry 1k26 coloured by chain, front view.
PDB entry 1k26 coloured by chain, side view.
PDB entry 1k26 coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
Structure of a Nudix protein from Pyrobaculum aerophilum reveals a dimer with two intersubunit beta-sheets.
Wang S, Mura C, Sawaya MR, Cascio D, Eisenberg D
Acta Crystallogr D Biol Crystallogr 58 571-8 (2002)
PMID: 11914479

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nudix hydrolase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.81 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZTD8 (Residues: 6-146; Coverage: 97%)
Gene name: PAE3301
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

5 bound ligands:
Ligand SO4 2 x SO4
Ligand IR3 4 x IR3
Ligand NI 2 x NI
Ligand GOL 3 x GOL
Ligand ACY 5 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 52.424Å b: 71.655Å c: 85.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structures and mechanisms of Nudix hydrolases.
Mildvan et al. (2005)
1 more

1 mention without citation

Stretching to understand proteins - a survey of the protein data bank.
SuĊ‚kowska et al. (2008)