› 1k2v

X-ray diffraction
1.97Å resolution

E. COLI PERIPLASMIC PROTEIN FHUD COMPLEXED WITH DESFERAL

Released:
DOI: 10.2210/pdb1k2v/pdb
PDB 1k2v front image not found
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Source organism: Escherichia coli
Primary publication:
X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin.
Clarke TE, Braun V, Winkelmann G, Tari LW, Vogel HJ
J Biol Chem 277 13966-72 (2002)
PMID: 11805094

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron(3+)-hydroxamate-binding protein FhuD Chain: N
Molecule details ›
Chain: N
Length: 266 amino acids
Theoretical weight: 29.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07822 (Residues: 31-296; Coverage: 100%)
Gene names: JW0148, b0152, fhuD
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand DEF 1 x DEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P63
Unit cell:
a: 85.57Å b: 85.57Å c: 91.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Bacterial iron homeostasis.
Andrews et al. (2003)
24 more

2 mentions without citation

Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.
Zawadzka et al. (2009)
1 more