› 1k2y

X-ray diffraction
1.75Å resolution

Crystal Structure of Phosphomannomutase/Phosphoglucomutase S108A mutant from P. aeruginosa

Released:
DOI: 10.2210/pdb1k2y/pdb
PDB entry 1k2y coloured by chain, front view.
PDB entry 1k2y coloured by chain, side view.
PDB entry 1k2y coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of PMM/PGM: an enzyme in the biosynthetic pathway of P. aeruginosa virulence factors.
Regni C, Tipton PA, Beamer LJ
Structure 10 269-79 (2002)
PMID: 11839312

Function and Biology Details

Reactions catalysed: 
Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphomannomutase/phosphoglucomutase Chain: X
Molecule details ›
Chain: X
Length: 463 amino acids
Theoretical weight: 50.33 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26276 (Residues: 1-463; Coverage: 100%)
Gene names: PA5322, algC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 71.064Å b: 71.306Å c: 96.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

The Pseudomonas aeruginosa AlgZR two-component system coordinates multiple phenotypes.
Okkotsu et al. (2014)
1 more

11 mentions without citation

Detecting coevolution in and among protein domains.
Yeang et al. (2007)
10 more