› 1k3i

X-ray diffraction
1.4Å resolution

Crystal Structure of the Precursor of Galactose Oxidase

Released:
DOI: 10.2210/pdb1k3i/pdb
PDB entry 1k3i coloured by chain, front view.
PDB entry 1k3i coloured by chain, side view.
PDB entry 1k3i coloured by chain, top view.
Source organism: Fusarium sp.
Primary publication:
Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme.
Firbank SJ, Rogers MS, Wilmot CM, Dooley DM, Halcrow MA, Knowles PF, McPherson MJ, Phillips SE
Proc Natl Acad Sci U S A 98 12932-7 (2001)
PMID: 11698678

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 70.32 KDa
Source organism: Fusarium sp.
Expression system: Aspergillus nidulans
UniProt:
  • Canonical: P0CS93 (Residues: 25-680; Coverage: 100%)
Gene name: GAOA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand CA 2 x CA
Ligand ACT 2 x ACT
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 69.313Å b: 89.516Å c: 93.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.193
Expression system: Aspergillus nidulans

Quick links

Citations

11 review citations

Structural and functional diversity of lectin repertoires in invertebrates, protochordates and ectothermic vertebrates.
Vasta et al. (2004)
10 more

7 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
6 more