› 1k47

X-ray diffraction
2.42Å resolution

Crystal Structure of the Streptococcus pneumoniae Phosphomevalonate Kinase (PMK)

Released:
DOI: 10.2210/pdb1k47/pdb
PDB entry 1k47 coloured by chain, front view.
PDB entry 1k47 coloured by chain, side view.
PDB entry 1k47 coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
Crystal structure of the Streptococcus pneumoniae phosphomevalonate kinase, a member of the GHMP kinase superfamily.
Romanowski MJ, Bonanno JB, Burley SK
Proteins 47 568-71 (2002)
PMID: 12001237

Function and Biology Details

Reaction catalysed: 
ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphomevalonate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 337 amino acids
Theoretical weight: 37.71 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8DR49 (Residues: 1-335; Coverage: 100%)
Gene names: mvaK2, spr0340
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P63
Unit cell:
a: 231.606Å b: 231.606Å c: 88.587Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
4 more

6 mentions without citation

Structural genomics: a pipeline for providing structures for the biologist.
Chance et al. (2002)
5 more