› 1k50

X-ray diffraction
1.8Å resolution

A V49A Mutation Induces 3D Domain Swapping in the B1 Domain of Protein L from Peptostreptococcus magnus

Released:
DOI: 10.2210/pdb1k50/pdb
PDB entry 1k50 coloured by chain, front view.
PDB entry 1k50 coloured by chain, side view.
PDB entry 1k50 coloured by chain, top view.
Source organism: Finegoldia magna ATCC 29328
Primary publication:
Single-site mutations induce 3D domain swapping in the B1 domain of protein L from Peptostreptococcus magnus.
O'Neill JW, Kim DE, Johnsen K, Baker D, Zhang KY
Structure 9 1017-27 (2001)
PMID: 11709166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-175983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 63 amino acids
Theoretical weight: 6.86 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51912 (Residues: 111-173; Coverage: 9%)
Sequence domains: Protein L b1 domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43
Unit cell:
a: 53.134Å b: 53.134Å c: 115.514Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The unfolding story of three-dimensional domain swapping.
Rousseau et al. (2003)
5 more

4 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
3 more