› 1k53

X-ray diffraction
2.1Å resolution

Monomeric Protein L B1 Domain with a G15A Mutation

Released:
DOI: 10.2210/pdb1k53/pdb
PDB entry 1k53 coloured by chain, front view.
PDB entry 1k53 coloured by chain, side view.
PDB entry 1k53 coloured by chain, top view.
Source organism: Finegoldia magna ATCC 29328
Primary publication:
Single-site mutations induce 3D domain swapping in the B1 domain of protein L from Peptostreptococcus magnus.
O'Neill JW, Kim DE, Johnsen K, Baker D, Zhang KY
Structure 9 1017-27 (2001)
PMID: 11709166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.07 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51912 (Residues: 111-173; Coverage: 9%)
Sequence domains: Protein L b1 domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 66.511Å b: 66.511Å c: 109.192Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.213 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The unfolding story of three-dimensional domain swapping.
Rousseau et al. (2003)
6 more

4 mentions without citation

In silico protein design by combinatorial assembly of protein building blocks.
Tsai et al. (2004)
3 more