› 1k63

X-ray diffraction
1.8Å resolution

Complex of hydrolytic haloalkane dehalogenase linb from sphingomonas paucimobilis with UT26 2-BROMO-2-PROPENE-1-OL at 1.8A resolution

Released:
DOI: 10.2210/pdb1k63/pdb
PDB entry 1k63 coloured by chain, front view.
PDB entry 1k63 coloured by chain, side view.
PDB entry 1k63 coloured by chain, top view.
Source organism: Sphingomonas paucimobilis
Primary publication:
Haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26: X-ray crystallographic studies of dehalogenation of brominated substrates.
Streltsov VA, Prokop Z, Damborský J, Nagata Y, Oakley A, Wilce MC
Biochemistry 42 10104-12 (2003)
PMID: 12939138

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.01 KDa
Source organism: Sphingomonas paucimobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 2-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand BR 2 x BR
Ligand CL 2 x CL
Ligand MG 3 x MG
Ligand BRP 2 x BRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 46.18Å b: 68.951Å c: 80.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.141 0.2
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.
Lal et al. (2010)
6 more

2 mentions without citation

CAVER: a new tool to explore routes from protein clefts, pockets and cavities.
Petrek et al. (2006)
1 more